Perforin-2 (MPEG1) knockout mice are not resistant to LPS induced septic shock.

In this animal model LPS is injected to induce sterile septic shock. C57BL/6 and 129X1/SvJ wild-type and perforin-2 (mpeg1) knockout mice were injected intraperitoneally with LPS from E. coli 0111:B4 (InvivoGen LPS-EB ultra-pure, catalog # tlrl-3pelps). Mice were monitored hourly for signs of morbidity and humanely euthanized as per IACUUC protocol. "1" indicates subject death, "0" indicates censored subject. On either genetic background the differences between survival rates of WT and perforin-2 KO animals are insignificant. Crucially, we did observe that 129 mice were more resistant to LPS challenge than BL6 mice. This was expected due to the caspase 11 mutation carried by 129s. All animal experiments were approved by and performed in accordance with the University of Miami Institutional Animal Care and Use Committee guidelines.Experiment 1. LPS challenge of WT and perforin-2 (mpeg1) -/- C57BL/6 and 129X1/SvJ miceExperiment 2. LPS challenge of WT and perforin-2 (mpeg1) -/- 129X1/SvJ mice