Transcriptomic Comparison of Common Nontyphoidal Salmonella Serovars Provides Greater Resolution for Understanding Differences in Virulence

RNA extracted from representative isolates of each serovar grown to late exponential phase in Luria-Bertani broth showed that transcript abundances of core genes were significantly higher (p < 0.05) than those of accessory genes for all three serovars. Inter-serovar comparisons demonstrated higher transcript abundances for genes related to vitamin B12 biosynthesis, and ethanolamine and 1,2-propanediol utilization in S. Cerro compared to Javiana and Typhimurium, while genes in Salmonella Pathogenicity Island (SPI) 1 had significantly higher transcript abundances in S. Javiana and S. Typhimurium. Overall design: mRNA profiles from three Salmonella serovars (Cerro, Javiana, Typhimurium) were generated by deep sequencing with an Illumina NextSeq 500 in triplicate with 75-nt stranded single-end reads.