Retrospective analysis of mesendoderm progenitors that give rise to cardiac microtissues, multilineage and gut organoids

Bulk RNA-sequencing of the mesendoderm progenitors before aggregation. Mesendoderm progenitors aggregates can lead to the formation of cardiac microtissues, multilineage and gut organoids in different batches. Despite batch-to-batch differences in spheroid phenotype, all spheroids (n>200) within a single batch derived from the same initial differentiation of progenitor cells manifested identical morphologies and cellularity. Mesendoderm progenitors at differentiation day 5.