Table 1_Suppressed oncogenic molecules involved in the treatment of colorectal cancer by fecal microbiota transplantation.docx

Dysbiosis of the intestinal microbiota is prevalent among patients with colorectal cancer (CRC). This study aims to explore the anticancer roles of the fecal microbiota in inhibiting the progression of colorectal cancer and possible mechanisms. The intestinal microbial dysbiosis in CRC mice was significantly ameliorated by fecal microbiota transplantation (FMT), as indicated by the restored ACE index and Shannon index. The diameter and number of cancerous foci were significantly decreased in CRC mice treated with FMT, along with the restoration of the intestinal mucosal structure and the lessening of the gland arrangement disorder. Key factors in oxidative stress (TXN1, TXNRD1, and HIF-1α); cell cycle regulators (IGF-1, BIRC5, CDK8, HDAC2, EGFR, and CTSL); and a critical transcription factor of the innate immune signal pathway (IRF5) were among the repressed oncogenic targets engaged in the FMT treatment of CRC. Correlation analysis revealed that their expressions were positively correlated with uncultured_bacterium_o_Mollicutes_RF39, Rikenellaceae_RC9_gut_group, and negatively correlated with Bacillus, Marvinbryantia, Roseburia, Angelakisella, Enterorhabdus, Bacteroides, Muribaculum, and genera of uncultured_bacterium_f_Eggerthellaceae, uncultured_bacterium_f_Xanthobacteraceae, Prevotellaceae_UCG-001, uncultured_bacterium_f_Erysipelotrichaceae, uncul-tured_bacterium_f_Lachnospiraceae, uncultured_bacterium_f_Ruminococcaceae, Eubacterium_coprostanoligenes_group, Ruminococcaceae_UCG-005, and uncultured_bacterium_f_Peptococcaceae. This study provides more evidence for the application of FMT in the clinical treatment of CRC.

肠道微生物群失调在结直肠癌（CRC）患者中普遍存在。本研究旨在探讨粪便微生物群在抑制结直肠癌进展及其潜在机制中的抗癌作用。通过恢复ACE指数和Shannon指数，粪便微生物群移植（FMT）显著改善了CRC小鼠的肠道微生物群失调。CRC小鼠经FMT治疗后，癌灶的直径和数量显著减少，同时肠道黏膜结构得到恢复，腺体排列紊乱减轻。氧化应激的关键因素（TXN1、TXNRD1和HIF-1α）；细胞周期调节因子（IGF-1、BIRC5、CDK8、HDAC2、EGFR和CTSL）；以及先天免疫信号通路的关键转录因子（IRF5）均参与了FMT治疗CRC的抗癌目标，其表达与uncultured_bacterium_o_Mollicutes_RF39、Rikenellaceae_RC9_gut_group呈正相关，与Bacillus、Marvinbryantia、Roseburia、Angelakisella、Enterorhabdus、Bacteroides、Muribaculum以及uncultured_bacterium_f_Eggerthellaceae、uncultured_bacterium_f_Xanthobacteraceae、Prevotellaceae_UCG-001、uncultured_bacterium_f_Erysipelotrichaceae、uncultured_bacterium_f_Lachnospiraceae、uncultured_bacterium_f_Ruminococcaceae、Eubacterium_coprostanoligenes_group、Ruminococcaceae_UCG-005和uncultured_bacterium_f_Peptococcaceae属等呈负相关。本研究为FMT在CRC临床治疗中的应用提供了更多证据。