Transcriptional profiling of conditional knockout mice lacking Izumo1r in FoxP3+ Tregulatory cells

We reported transcriptional characterization of splenic, ear skin and back skin of Izumo1R flox X Foxp3Cre mice and littermate controls. We report few changes observed in splenic Tregs but changes seen in the whole skin transcriptome and gamma delta compartment starting at approximately 8 weeks of age. We report upregulation of inflammatory genes overlapping with genes upregulated in the skin following topical administration of Imiquimod. For the IMQ treated samples, dorsal and ventral sides of the ear were treated with 125ug of 5% Imiquimod cream until indicated date. All other data are from untreated mice at indicated age. Foxp3/GFP+ Treg cells were sorted and processed for RNA-Seq. TCRB+ CD4+ Foxp3GFP- cells were sorted from the skin as Tcon. TCRb-CD19-GammaDeltaTCR+ cells from the skin were sorted as skin gamma delta T cells. All sorted samples were pre-gated on DAPI- single cells.