A library of reporters of the global regulators of gene expression of Escherichia coli

The topology of the transcription factor network (TFN) of E. coli is far from uniform, with 22 global regulator (GR) proteins controlling one-third of all genes. So far, their production rates cannot be tracked by comparable fluorescent proteins. We developed a library of fluorescent reporters for 16 GRs. Each consists of a single-copy plasmid coding for GFP fused to the full-length copy of the native promoter. We tracked their activity in exponential and stationary growth, and under weak and strong stresses. We show that the reporters have high sensitivity and specificity to all stresses tested and detect single-cell variability in transcription rates. Given the influence of GRs on the TFN, we expect that the new library will contribute to dissecting global transcriptional stress-response programs of E. coli. Moreover, it can be invaluable in bio-industrial applications that tune those programs to, instead of cell growth, favor productivity while reducing energy consumption., , , # A library of reporters of the global regulators of gene expression of *Escherichia coli*

**Recommended citation for this dataset:** S. Dash, R. Jagadeesan, I.S.C. Baptista, V. Chauhan, V. Kandavalli, S.M.D. Oliveira and A.S. Ribeiro. (2024). A library of reporters of the global gene expression regulators of *Escherichia coli*; Dryad (dataset)

## DATA & FILE OVERVIEW

### Flow\_Cytometry

* This folder contains all flow cytometry data used in the project. The folder structure is organized as a function of the figures in the paper.
* 01_Optimal_Conditions: Related to Figure 1C and 4A, 4B of main manuscript
* 02_Strong_Stress: Related to Figure 6(A-D) and of main manuscript and Figure S11 of Supplementary information
* Note: All flow cytometry data was acquired using an ACEA NovoCyte Flow Cytometer (ACEA Biosciences Inc., San Diego, USA). All flow cytometry raw data is provided as *.csv files. The units are in arbitrary units (a.u.). Each raw file contains 26 columns, out ...