Overexpression of sli-1 reverses the effect of sli-1(sy143) on engulfment in ced-10(n1993); sli-1(sy143) animals.

ced-10(n1993); sli-1(sy143) embryos containing the transgenes indicated above were heat shocked at 33°C for 1 hour and then allowed to recover for 3–3.5 hours at 20°C. The numbers of cell corpses in the heads of anaesthetized first larval stage (L1) worms were counted using DIC microscopy within 0.5 h of hatching. The number of persistent corpses was determined from two independent transgenic lines for each transgene except for Phspsli-1ΔRING (see text for explanation). s.d., standard deviation.