The disease resistance genes encoding NBS-LRR proteins in the Solanaceae family, mainly tomatoes, were selectively captured, enriched and sequenced using resistance gene enrichment sequencing (RenSeq) and PacBio.. Resistance gene enrichment sequencing (RenSeq) of tomatoes

Resistance gene enrichment sequencing (RenSeq) enables selective capture and enrichment of the genes that encode nucleotide-binding sites and leucine rich repeats (NBS-LRR) proteins, major components of plant immunity. RenSeq was applied mainly to S. lycopersicum Heinz and wild tomato speices as well as N. benthamiana and C. annuum which all belong to Solanaceae. The enriched libraries were sequenced using PacBio Sequel.