Genome wide analysis of overexpression of miR-203 in primary mouse cortical neurons

Effect of lentiviral mediated overexpression of miR-203 in mouse cortical neurons at different days in vivo Total RNA isolated from E15 cortical cultures using QIAGEN miRNeasy mini kit (QIAGEN; 217004) were processed on an Illumina mouse Ref8 v2 beadchip microarrays following the manufacture’s protocol. Microarray data analysis was performed using R and Bioconductor packages. Raw expression data were log2 transformed and normalized by quantile normalization. Probes were considered robustly expressed if the detection P value was 0.05 for at least half of the samples in the data set. Probes were re-mapped to mouse Ensembl gene IDs (v67; May 2012 data freeze) for comparison with RNA-sequencing data. Mouse primary cortical neurons were overexpressed with miR-203 or scrambled miRNA (Control) using lentiviral construct. Untreated cells were used as unt controls. Cells were infected at DIV0 and RNA isolated at various time points - DIV6, DIV8, DIV10 and DIV13. 4 independent replicates (biological) were used for the study.