Intracellular concentrations of TYMS mRNA and hTS.

*Using an estimated cell volume of 1.86*10−12 L.Intracellular concentrations of hTS protein and TYMS mRNA are indicated. The amounts of hTS protein (figure S2) and TYMS mRNA in the pull down fraction after immunoprecipitation are also shown. Antibody against Beta-tubulin was used to evaluate the non-specific binding between TYMS mRNA and a generic protein. The non-specific interaction between mRNAs and hTS protein was checked by quantification of GAPDH mRNA bound to hTS in the pull down fraction. (Intracellular concentration: n = 5, error = ±S.D. Pull down amount: n = 2, error = ±S.E.)