Expression data from healthy (control) and Staph sureus mastitis cows' mononucle

Analysis of gene expression changes in blood mononuclear cells (BMCs) that occur with Staph Aureus mastitis. We used in house microarrays to indicate the changes that occur in gene expression in the BMCs as a result of mastitis Keywords: Comparison mastitis animal vs control animal Blood was taken from tail vein of 7 pairs of Holstein cows identified as chronic S. aureus carriers (mastitis susceptible) and the ages, herds, stage of lactation and parity-matched control cows with no evidence or previous history of S. aureus mastitis. Total RNA was extracted and reverse transcripted to cDNA. Labelled cDNA probes (Cy3 and Cy5) derived from BMCs and MSCs) of infected quarters and healthy quarters of controls were hybridised to a bovine immune-endocrine cDNA array containing immune and endocrine related genes and the resulting changes in gene expression were analysed.