In vitro RuBisCO partial digest for In vivo transformations of positively charged nanoparticles alter the formation and function of RuBisCO photosynthetic protein corona manuscript

Peptide samples were first lyophilized followed by resuspending in 0.1% trifluoroacetic acid (TFA) and cleaned and concentrated using StageTips. Samples were then lyophilized again and resuspended in 0.1% formic acid (FA)/ 5% acetonitrile (ACN) followed by injecting into a UltiMateTM 3000 RSLCnano System coupled to a Q Exactive HF-X mass spectrometer.