Summary on the evaluation of DM primers during DHPLC and SN/WAVE-HS analyses.

For DHPLC analysis, Y indicates the presence of small but clear heteroduplex peaks, whereas N indicates the absence of such peaks. The question mark indicates the presence of ambiguous shoulder peaks. For SN/WAVE-HS analysis, Y in the uncut groups indicates the presence of cleaved DNA fragments of sizes different from those of the undigested peak, whereas Y in the cut vs. uncut column indicates changes in patterns of peaks from cleaved fragments after SN digestion. ND indicates that the experiments were not performed for cybrid cells because there was no positive signal or change in the chromatograms of 143B cells.