RNA microarray analysis of Human Umbilical Vein Endothelial Cells in normal pregnancy and preeclampsia

To investigate the differentially expressed lncRNAs,CircRNAs and mRNAs in Human Umbilical Vein Endothelial Cells between normal pregnancy and preeclampsia, we performed the human lncRNA,cirRNA and mRNA microarray analysis of 8 samples from clinical patients. Ten preeclampsia patients (mean age 33.1 ± 1.84 years) and ten control patients (mean age 30.4 ± 1.36 years) who received a caesarean section were included in this study. Preeclampsia was clinically diagnosed before caesarean. The main criteria for PE diagnosis were: SBP ≥ 140 or DBP ≥ 90 on two occasions at least 4 h apart; and proteinuria >0.3 g/24h. The control pregnancy was defined as: no medical complications and proteinuria, maternal blood pressure < 140/90 mm Hg. Of the ten pairs of placenta samples, four pairs were randomly selected for lncRNA, circRNA and mRNA microarray analysis, and the other samples were used for the following verification.