Effects of TNF-alpha blocking in sorted Th1 cells from co-cultures of human CD4-positive and CD14-positive cells

Human CD4+ T cells and CD14+ monocytes from healthy donors were co-cultured with anti-CD3 for three days in the presence or absence of TNF-alpha mAb (Adalimumab). Classical Th1 cells or those generated in the presence of the inhibitor were then sorted and analyzed by full transcriptome microarray analysis. Sorted Th1-cells from co-cultures of CD4+ T-cells and CD14+ monocytes (+/- Adalimumab) were prepared from 8 healthy volunteers. Total RNA was isolated and profiled using Affy HG-U219 chips.