Effect of 1-25D and androgens on junctional transfer of fluorescent tracers in LNCaP-32 cells under androgen-depleted conditions.

LNCaP-32 cells were seeded as described in Table 1 legend. Cells were switched to charcoal-stripped, androgen-depleted medium (Strip) for 48 h in the presence and absence of 1-25D and synthetic (MB) and the natural (DHT) androgens.aThe number of fluorescent cell neighbors (mean ± SE) 1 min (Lucifer Yellow), 3 min (Alexa-488) and 15 min (Alexa-594) after microinjection into test cell. The total number of injection trials is shown in parentheses.bCells were stripped for 24 h in the presence and absence of 1-25D, DHT (10 nM) and MB (2.5 nM).cP≤0.0001 for normal serum (NS) versus stripped (Strip) for all tracers. A two tailed Student's t test was used to calculate P value assuming unequal variance.dP≤0.0001 for stripped serum (Strip) versus stripped and treated cells for all the tracers. A two tailed Student's t test was used to calculate P value assuming unequal variance.Effect of 1-25D and androgens on junctional transfer of fluorescent tracers in LNCaP-32 cells under androgen-depleted conditions.