Identifying RNAs bound by Drosophila Zn72D

Zn72D is an RNA binding protein that alters A-to-I RNA editing and splicing in the fly brain. We performed RNA immunoprecipitation and sequencing (RIP-seq) to identify the RNAs that are bound by Zn72D. We immunprecipitated Zn72D using an anti-GFP antibody in Zn72D-GFP(BDSC50830) fly heads. We extracted RNA from 5% of input lysates and after immunoprecipitation from three replicates total.