Rachel Horowitz-Scherer, Chris Woodcock (2010) CIL:771, Patiria miniata, sperm. CIL. Dataset

Phosphorus has a characteristic electron energy loss with an edge at 160 eV. The Zeiss 902 electron microscope permits images to be recorded at different energy losses which can be digitized and processed to yield a net phosphorus image. For nuclear chromatin, phosphorus is primarily located in DNA, allowing its distribution in chromatin fibers to be mapped. Electron energy loss (EELS) imaging is also known as electron spectroscopic imaging (ESI) and electron energy filtering TEM (EFTEM).
Starfish sperm chromatin, which has clearly defined chromatin fibers was fixed, embedded in Lowicryl at low temperature, thin sections prepared and imaged unstained. The 3 grouped images were recorded with a Zeiss 902 energy filtered TEM at 80KV using 5eV windows centered on energy loss values of 160, 120 (this image), and 104eV.

磷元素具有特有的电子能量损失，其边缘位于160电子伏特。蔡司902型电子显微镜允许记录不同能量损失下的图像，这些图像可以被数字化并处理，从而生成净磷图像。对于核染色质，磷主要位于DNA中，这允许其分布情况在染色质纤维中被绘制出来。电子能量损失成像（EELS）亦被称为电子能谱成像（ESI）以及电子能量过滤透射电镜（EFTEM）。海星精子的染色质，其染色质纤维清晰可辨，经过固定、低温Lowicryl包埋，制备了薄切片并进行无染色的成像。三个分组的图像使用蔡司902型能量过滤透射电镜在80KV下记录，使用5电子伏特窗口，窗口中心分别位于160、120（此图像）和104电子伏特的能量损失值处。