Detection of humoral responses induced by various hematophagous insects.

Serially diluted plasmas of control mice and mice repeatedly exposed to the bites of G. morsitans morsitans, G. fuscipes fuscipes, G. palpalis gambiensis and G. pallidipes (n = 5/group) were tested in the G. m. morsitans saliva-based (A) and rTsal1-based IgG detection ELISA (B). Serially diluted plasmas of control mice and mice experimentally immunized with harvested G. m. morsitans, Stomoxys calcitrans and Tabanus yao saliva (n = 6/group) were analysed for responses against the respective saliva extracts (C) and were tested in the G. m. morsitans saliva-based (D) and rTsal1-based IgG detection ELISA (E). Presented data are the mean ΔO.D.450 nm values with the 95% CI.