Purification of recombinant mAOX1 after expression in E. coli TP1000 cells.
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aTotal protein was quantified with the Bradford assay.bThe activity was measured by monitoring the decrease in absorption at 600 nm in the presence of 500 µM benzaldehyde and 100 µM DCPIP.cSpecific enzyme activity (units/mg) is defined as the oxidation of 1 µM benzaldehyde per min and mg of enzyme under the assay conditions.
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2015-12-02
