T. gondii Genome Wide CRISPR Screen for Fitness-Conferring Genes. Toxoplasma gondii

We provide examples of the next-generation sequencing results obtained after analyzing sgRNAs (single guide RNAs) isolated from an input library and from parasites three passages after transfection. Overall design: sgRNAs were isolated from parasites that had been passaged in human foreskin fibroblasts three times after transfecting the sgRNA library. The abundances of the sgRNAs can be compared to those in the input library (also provided). sgRNAs that are lost over time are presumed to cleave genes that confer fitness.