Parallel neural pathways control sodium consumption and taste valence

We performed single-cell RNA-seq (10x Chromium 3' v.3.1) on dissociated single cell suspensions from mouse (C57BL/6) osmosensory brain nuclei - subfornical organ (SFO) to determine transcriptomic cell types and neuronal activation patterns. By performing single cell RNA-seq based stimulus to cell-type mapping, we captured the endogenous immediate early gene expression triggered by distinct physiological state stimuli in SFO cells. We identified a unique excitatory neuron type in SFO that is uniquely tuned to sodium deficiency.我们对来自C57BL/6小鼠渗透压感受脑核——穹窿下器（subfornical organ, SFO）的解离单细胞悬液进行了单细胞RNA测序（single-cell RNA-seq，10x Chromium 3' v.3.1），以确定其转录组细胞类型与神经元激活模式。通过开展基于单细胞RNA测序的刺激-细胞类型映射分析，我们捕获了SFO细胞中由不同生理状态刺激触发的内源性即刻早期基因表达情况。我们在SFO中鉴定出一种独特的兴奋性神经元类型，该类型仅特异性响应钠缺乏。