Use of genetics in monitoring control measures for Aedes aegypti and Aedes albopictus

This study evaluated the impact of long-term pyriproxyfen exposure on the genetic structure and diversity of Ae. aegypti and Ae. albopictus mosquito populations. The study was conducted in Manaus, Amazonas, Brazil, where pyriproxyfen dissemination stations have been monitored since 2014 up to the present day. Double digest restriction-site associated DNA sequencing (ddRADSeq) was performed. Sequencing quality control was applied with mean quality filtering, trimming, minimum length filtering and adapter removal procedures using Trimmomatic. All sequencing reads were aligned with BWA program to the reference genome of Ae. aegypti (NC_035159.1) and Ae. albopictus (JAFDOQ000000000.1). Variant calling was performed using Stacks v2.62, with gstacks approach. The variant panel was exported in the VCF v4.2 format. SNPs were removed from the variant panel based on a minimum allele frequency (MAF) < 0.1 and at least 8 non-missing genotypes within each group.