Proxy component spectra

This is a WiTec Project file with spectra obtained under the same conditions as for biofilm measurements, of pure proxy components with colloidal Ag. From M&M: In order to identify the individual SERS spectra of pertinent components of the S. oneidensis biofilm, we first performed SECRaM analysis on several component proxies mixed with colloidal silver (Sigma Aldrich, Hamburg, Germany). The proxy component-colloidal silver mixture was analyzed under the same working conditions as the ones applied for biofilm analysis. The same excitation wavelength, laser intensity and setup were used, however without a Ag/AgCl patch. This was done, separately, for the following expected component proxies: horse heart cytochrome c (hhcytc) (Sigma Aldrich, Hamburg, Germany) in Phosphate buffer solution (PBS) (Roth, Karlsruhe, Germany); riboflavin phosphate (Sigma Aldrich, Hamburg, Germany) in PBS; hhcytc in PBS with excess sodium dithionite (Merck, Darmstadt, Germany); hhcytc in PBS with excess potassium hexacyanoferrate (III) (Sigma Aldrich, Hamburg, Germany); riboflavin phosphate in PBS with excess sodium dithionite; riboflavin phosphate in PBS with excess potassium hexacyanoferrate; an aqueous solution of sodium alginate (Sigma Aldrich, Hamburg, Germany); pure PBS; PBS with sodium dithionite; and PBS with potassium hexacyanoferrate. Sodium dithionite and potassium hexacyanoferrate, respectively, were used to obtain reduced and oxidized cytochrome and flavin species.