Genome-wide analysis of Dnmt3L heterozygous and wildtype spermatocytes and spermatids

Comparison of gene expression differences between Dnmt3L heterozygous and wildtype pachytene spermatocytes, and similarly between Dnmt3L heterozygous and wildtype round spermatids which were isolated from the Dnmt3L knockout mouse line. This array was conducted to address the hypothesis that Dnmt3L heterozygosity results in deregulated gene expression within spermatocytes and spermatids. Results show that Dnmt3L heterozygosity causes numerous genes to be differentially regulated on a genome-wide level, showing that DNMT3L has an important role in regulating gene expression within these male germ cells. Three preparations each of Dnmt3L purified wildtype spermatocytes, wildtype spermatids, heterozygous spermatids, and two preparations of heterozygous spermatocytes were isolated for a total of 11 samples. Each preparation was made up of cells isolated from 10 mice.本数据集旨在比较Dnmt3L杂合型与野生型粗线期精母细胞（pachytene spermatocytes）的基因表达差异，并对从Dnmt3L基因敲除小鼠品系（Dnmt3L knockout mouse line）中分离得到的Dnmt3L杂合型与野生型圆形精子细胞（round spermatids）进行同等类型的比较。本基因表达芯片（array）实验旨在验证下述假说：Dnmt3L杂合性会导致精母细胞与圆形精子细胞内的基因表达失调。实验结果显示，Dnmt3L杂合性可在全基因组水平引发大量基因的差异表达调控，证实DNMT3L在这类雄性生殖细胞（male germ cells）的基因表达调控中发挥重要作用。本次实验共制备11份样本：经Dnmt3L纯化的野生型精母细胞、野生型圆形精子细胞、杂合型圆形精子细胞各3份样本，杂合型精母细胞制备2份样本；每份样本均由10只小鼠分离得到的细胞混合制备而成。