Short C-terminal Musashi-1 proteins regulate pluripotency states in embryonic stem cells

To understand the function of MSI1 in pluripotent stem cells, RNA-seq assays were performed on mouse embryonic stem cells R1, MSI1 knockout cell line R1-C5, human embryonic stem cells H9, RRM knockout cell line H9-C8, MSI1 full-length overexpression cell line H9-MSI1OE, MSI1C variant overexpression cell line H9-MSI1 (138-362) OE , H9-MSI1(272-362)OE. RNA bound by MSI1 in R1 and H9, and MSI1C variants MSI1 (138-362), MSI1(272-362) were detected using RIP-seq. RNA-seq for mESC cell line R1 (R1-WT and MSI1-KO cell line R1-C5 ), hESC cell line H9 (H9-WT, MSI1 RRM KO cell line H9-C8, MSI1 overexpression cell H9-MSI1FL and MSI1-C variant overexpression cell H9-MSI1V138 and H9-MSI1V272). RIP-seq for MSI1 binding RNA in R1 and H9 by using anti-MSI1 antibody for RIP. RIP-seq for MSI1-C variants binding RNA in H9 by using anti-Flag antibody for RIP.