SIRT6-dependent gene transcriptional profile in human endothelial cells

Sirtuin 6 (SIRT6), a well-recognized longevity gene, regulates genome stabilization, DNA repair, inflammation, and metabolic homeostasis. SIRT6 expression is decreased in atherosclerotic lesions from apolipoprotein E deficient mice and human patients. SIRT6 expression is also decreased in endothelial cells (ECs) under chronic stimulation with lipopolysaccharide, hydrogen peroxide and high glucose, three of which are risk factors associated with endothelial dysfunction and atherogenesis. To gain mechanistic insights into the effects of SIRT6 on endothelial gene transcriptome, we performed RNA-sequencing (RNA-seq) analysis of control adenovirus and SIRT6 adenovirus infected human umbilical vein endothelial cells (HUVECs). Gene ontology (GO), pathway enrichment and functional annotation clustering analysis were performed to classify differentially expressed genes and to identify the most significantly enriched GO terms/pathways/gene clusters. Human Umbilical Vein Endothelial Cells (HUVEC, passage 3 to passage 5) were seeded in 0.2% gelatin-coated dishes the day before experiment. HUVECs at 100% confluence were infected with control adenoviral LacZ or adenoviral SIRT6 for 48 h.