A revised phylogeny of Boletaceae using whole genome sequences

The porcini mushroom family Boletaceae is a diverse, widespread group of ectomycorrhizal (ECM) mushroom-forming fungi that so far has eluded intrafamilial phylogenetic resolution based on morphology and multi-locus datasets. In this study, we present a genome-wide molecular dataset of 1764 single-copy gene families from a global sampling of 418 Boletaceae specimens. The resulting phylogenetic analysis has strong statistical support for most branches of the tree, including the first statistically robust backbone. The enigmatic Phylloboletellus chloephorus from non-ECM Argentinian subtropical forests was recovered as a new subfamily sister to the core Boletaceae. Time-calibrated branch lengths estimate that the family first arose in the early- to mid-Cretaceous and underwent a rapid radiation in the Eocene, possibly when the ECM nutritional mode arose with the emergence and diversification of ECM angiosperms. Biogeographic reconstructions reveal a complex history of vicariance and episodi..., These are genome assemblies produced from Illumina 150bp paired end sequences. Sequences were quality pruned and adapter trimmed with fastp, then assemblied with SPADES v3.15.0. Assembly quality was assessed with BUSCO., , # Genome assemblies of Boletaceae specimens from Tremble et al 2024

[https://doi.org/10.5061/dryad.dv41ns25n](https://doi.org/10.5061/dryad.dv41ns25n)

Sequences are DRAFT genome assemblies of Boletaceae specimens, assembled from ~50x Illumina 150bp paired-end sequences with SPADES. It is important to note that gDNA for these specimens was extracted from sporocarps, and thus may contain some non-endogenous bacterial contigs. That being said, in our experience non-endogenous DNA is very rarely widely abundant in these meta-genomes (For example, 1 out of 150 genomes had exhibited bacterial predicted proteins in its proteome... see Tremble et al. 2024 [https://doi.org/10.1101/2023.09.05.556370)](https://doi.org/10.1101/2023.09.05.556370\))

Scaffolds have been sorted and renamed by size, with the naming convention of >SAMPLEID_scaffold_X (X corresponds to scaffold ID)