Genome wide mRNA profiling of AGS, gastric cancer cell line upon treatment with 5µM of PD98059

ERK/MAPK pathway is one of the highly activated and a crucial player in gastric cancer proliferation and progression. To delineate the complex transcription program associated with ERK/MAPK pathway, PD98059 a known ERK/MAPK inhibitor was treated in AGS, one of the ERK/MAPK signaling pathway over expressed gastric cancer cell line. The resultant changes in genome-wide mRNA expression pattern between control and PD98059 treated was profiled using Human Gene 1.0 ST arrays. Total RNA was isolated from AGS gastric cancer cell line treated with 5µM of PD98059 and DMSO as a control and profiled using Affymetrix Human Gene 1.0 ST Array (HuGene-1_0-st).