Single-cell RNA-Sequencing maps the hematopoietic landscape in naked mole-rats [sorted_BM_PB_THY]

Naked mole-rats are a mammalian model organism of exceptional longevity. We mapped the hematopoietic hierarchy and used functional characterizations to define a purified stem and progenitor cell (HSPC) compartment similar to mouse LIN-/Sca-1+/Kit+ HSPCs or human LIN-/CD34+/CD38lo HSPCs. Naked mole-rat cells were sorted according to functionally characterized immunophenotypes as described in the manuscript, in parallel FACS-antibodies were conjugated to oligo-ADT tags to quantify surface marker binding as described for CITE-Seq. The mRNA libraries were aligned to either the FRAMA bone marrow transcriptome assembly from our publication or a mouse genome reference. Note: Raw data represents only gene expresion (GEX). Raw data for ADT not included.