mouse gut metagenome Raw sequence reads

In vitro incubations with fecal samples from human microbiota-associated mice (HMAM) under different diets. Six female C57BL/6 (B6) germ-free mice inoculated with fecal slurry from humans. Mice were maintained on a chow diet for 2 weeks after humanization, then, a diet high in fiber (Teklad 2018S) was administered for two weeks, and then switched to a low fiber diet for two weeks (Teklad TD.97184). Three fecal pellets were collected for each mouse after each experimental diet (high and low in fiber) and used separately as inoculum for in vitro incubations with quercetin (anaerobic 7N minimal medium plus acetate and 0.25 mg/ml of quercetin). Sampling was done at 0 and 7 days of incubation.