Transcriptome analysis of A549 cells upon LITATS1 knockdown

We identified a tumor-supressing long non-coding RNA (lncRNA) named lncRNA induced by TGF-beta and antagonizes TGF-beta signaling 1 (LITATS1). It was found to mitigate epithelial-to-mesenchymal transition (EMT), cell migration and extravasation in a zebrafish exnograft model. To better dissect the downstream target genes and elucidate the signaling pathways altered by LITATS1, we depleted LITATS1 by specific shRNAs in A549 cells. Total RNA was collected and sent for RNA sequencing analysis. Afterwards, differentially expressd genes were used for the pathway enrichment analysis and gene set enrichment analysis (GSEA). LITATS1 is depleted by two independent shRNAs in A549 cells. RNA from cells transduced with the non-targeting shRNA (shNT) was set as a control. Three biological replicates were included in each group.