Frontal Cortex whole transcriptome sequencing in COVID-19 elderly patients

SARS-Cov-2 infection is frequently associated with Nervous System (NS) manifestations. However, it is not clear how SARS-CoV-2 can cause neurological dysfunctions and which molecular processes affects. In this work, we examined the frontal cortex tissue of patients who died of COVID-19 for the presence of SARS-CoV-2, comparing qRT-PCR with ddPCR. We also investigated the transcriptomic profile of frontal cortex from COVID-19 patients and matched controls by RNA-seq analysis to characterize the transcriptional signature. Frontal cortex from 9 COVID-19 patients and 7 age- and sex-matched healthy controls (CTRL) were deep-sequenced and included in Real Time PCR experiments.