Mesenchymal cell-derived septoclasts resorb cartilage during endochondral ossification and fracture healing

For scRNA-seq of fracture bone , 14 days post-fracture (PFD14) femur and control bones were harvested, cleaned surrounding tissue and the nail insert was removed from fracture bone. Control and PFD14 bone single cell suspension and non-hematopoietic stromal cells were prepared for scRNA-seq . Fracture bone single cell -RNA sequencing of non-hematopoietic bone stromal cells (BSC) using 10X genomics