Aspirin-triggered Resolvin D1 Reduces Chronic Dust-induced Lung Pathology Without Altering Susceptibility to Dust-enhanced Carcinogenesis.

Farm workers are at an increased risk for the development of acute and chronic lung inflammatory diseases from their everyday exposures to organic dust. Previous investigations have examined the inflammatory effects in mice from single and repetitive exposures to dust from swine confinement facilities, however, no study has explored these effects in a chronic model. To address this research gap, we established a chronic dust exposure mouse model of lung tumor-igenesis that was also used to measure the efficacy of omega-3 fatty acid-derived lipid mediators as therapeutics for mitigating these induced responses. Our results from these investigations are the first to evaluate the chronic inflammatory, and carcinogenic effects of these dusts, as well as identify a potential therapeutic strategy for mitigating the inflammatory effects by using an omega-3 fatty acid-derived bioactive lipid mediator. Of the 65 total mice used in these studies, 24 left lung tissues were randomly selected among the saline-only, HDE-only, saline+NNK, and HDE+NNK-exposed groups, for transcript and pathway gene expression analyses using the NanoString platform. Left lung tissues were homogenized and RNA was extracted using the PureLink RNA Mini Kit (Invitrogen, Carlsbad, California, USA) and sample quality was quantified using the NanoDrop ND-100 (NanoDrop Technologies, Inc, Wilmington, DE, USA) and an Agilent 2100 Bioanalyzer (UC Riverside Core Facilities, Agilent Technologies, Santa Clara, CA, USA). We utilized the mouse PanCancer Immune Profiling Panel (NanoS-tring Technologies, Seattle, WA, USA) codeset which is a pre-designed panel containing 770 target genes related to immune response and carcinogenesis.