Cyanobacteria abundance, cyanotoxin concentration, and water quality data for the upper San Francisco Estuary, California, USA: 2014-2019

The goal of these measurements was to quantify Microcystis abundance and microcystin
concentration and associated water quality conditions during summer
blooms in the upper San Francisco Estuary in California, USA. Blooms
of harmful algae are a major ecological concern in the area because
harmful algae produce toxins and other metabolites, which
deteriorate water quality and negatively impact the aquatic
ecosystem. Our research team collected biological, physical, and
chemical data at 2-week to 4-week intervals during the summer and
fall from 2014 through 2019. Data included surface measurements of Microcystis volume (area-based diameter) by
microscopy (flowCAM digital imaging flow cytometry) and subsurface
(1 m depth) measurements of Microcystis , Aphanizomenon and Dolichospermum cell abundance measured by
quantitative PCR, cyanotoxin concentration (total microcystins,
anatoxin a and saxitoxin) measured by protein
phosphatase inhibition assay or enzyme linked immunosorbent assay,
and a suite of water quality parameters (water temperature,
dissolved oxygen, nutrient concentration, water transparency,
specific conductance, turbidity, pH, and chlorophyll a concentration). Details for the field
sampling and analytical methods are available in Lehman et al.
(2017). We also performed shotgun metagenomic analyses to
investigate biodiversity of cyanobacteria and other aquatic
microorganisms and all the DNA sequencing data are publicity
available (www.ncbi.nlm.nih. gov/; BioProject ID: PRJNA434758,
Kurobe et al. 2018, Lehman et al. 2021). During the study, we
experienced critically dry (2014 and 2015), below normal (2016 and
2018), and wet years (2017 and 2019), therefore data obtained in
this study provided a unique opportunity to assess impacts of
extreme conditions on the aquatic ecosystem (Kurobe et al. 2018,
Lehman et al. 2020).

本数据集的测量目标为定量分析美国加利福尼亚州旧金山上游河口夏季蓝藻水华期间微囊藻（Microcystis）丰度、微囊藻毒素浓度及相关水质状况。有害藻华是该区域主要的生态隐患，有害藻类可产生毒素及其他代谢产物，进而恶化水质并对水生生态系统造成负面影响。本研究团队于2014至2019年的夏季与秋季，以2至4周为采样间隔，采集了生物、物理及化学三类数据。采集的数据涵盖：通过显微镜结合flowCAM数字化成像流式细胞仪（flowCAM digital imaging flow cytometry）测定的微囊藻体积（基于面积的直径）表层测量值；采用定量PCR（quantitative PCR）测定的1米深度亚表层的微囊藻（Microcystis）、鱼腥藻（Aphanizomenon）与束丝藻（Dolichospermum）细胞丰度；通过蛋白磷酸酶抑制法或酶联免疫吸附试验（ELISA）测定的蓝藻毒素浓度（包括总微囊藻毒素、鱼腥藻毒素a与石房蛤毒素）；以及一系列核心水质参数：水温、溶解氧、营养盐浓度、水体透明度、比电导率、浊度、pH值与叶绿素a浓度。野外采样与分析方法的详细细节可参见Lehman等人（2017）的研究成果。本研究同时开展了鸟枪宏基因组分析，以探究蓝细菌及其他水生微生物的生物多样性；所有DNA测序数据均公开可获取（网址：www.ncbi.nlm.nih.gov/；生物项目编号：PRJNA434758，相关成果参见Kurobe等人2018、Lehman等人2021的研究）。本研究期间涵盖了极端干旱年份（2014、2015年）、降水低于正常水平的年份（2016、2018年）与多雨年份（2017、2019年），因此本研究获取的数据为评估极端水文条件对水生生态系统的影响提供了独特契机（相关分析参见Kurobe等人2018、Lehman等人2020的研究）。