Induced cell-autonomous neutropenia systemically perturbs hematopoiesis in Cebpa enhancer-null mice

Knockouts of the Cebpa gene or its +37kb enhancer in mice show two major findings: (1) neutropenia in bone marrow and blood; (2) decrease in long-term hematopoietic stem cell (LT-HSC) numbers. To study mechanisms linking the two observed events, we applied RNA sequencing on +37kbWT and +37kbHOM bone marrow cells. The analysis of these data revealed that myeloid priming in HSPCs occurs before Cebpa activation and that a neutrophil differentiation block in these progenitors potentially leads to HSC quiescence exit. Overall design: RNA-seq of hematopoietic stem and progenitor cells (HSPCs) isolated with LSK markers and common myeloid progenitors (CMP) from Cebpa null mice and wild type mice