Table S2. from Protein aggregation and the evolution of stress resistance in clinical yeast
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<b>Protein abundance in the aggregate fraction of [<i>RNQ</i><sup>+</sup>][<i>psi</i><sup>−</sup>], [<i>rnq</i><sup>−</sup>][<i>psi</i><sup>−</sup>], and [<i>RNQ</i><sup>+</sup>][<i>PSI</i><sup>+</sup>] in comparison with total proteomic data and stress granule proteome.</b> Protein abundance (measured in peptide spectra count “PSM” and expressed in parts per million “ppm”) in isolated aggregates from [<i>RNQ</i><sup>+</sup>][<i>PSI</i><sup>+</sup>], [<i>RNQ</i><sup>+</sup>][<i>psi</i><sup>−</sup>], and [<i>rnq</i><sup>−</sup>][<i>psi</i><sup>−</sup>] strains are tabulated in Total Protein sheet. The comparison between stress granule and p-body were tabulated in Stress Granule P-body sheet. The ribosomal proteins identified in aggregate from [<i>RNQ</i><sup>+</sup>][<i>PSI</i><sup>+</sup>], [<i>RNQ</i><sup>+</sup>][<i>psi</i><sup>−</sup>], and [<i>rnq</i><sup>−</sup>][<i>psi</i><sup>−</sup>] strains were tabulated in Ribosomal Proteins sheet. The known yeast abundance from unified whole proteome quantification was listed for comparison [17]. Both the total proteome dataset and the aggregate isolation described in here were obtained from cells harvested during mid-exponential phase in YPD medium. The abundance in parts per million for [<i>RNQ</i><sup>+</sup>][<i>PSI</i><sup>+</sup>], [<i>RNQ</i><sup>+</sup>][<i>psi</i><sup>−</sup>], and [<i>rnq</i><sup>−</sup>][<i>psi</i><sup>−</sup>] strains were calculated by normalizing spectral counts of individual proteins by total spectral counts per samples and multiplying one million. Columns with a “PSM-Sol” suffix was abundance measures for the fraction that was SDS-soluble whereas columns with a “PSM-Res” suffix was the fraction that was SDS-resistant.
提供机构:
The Royal Society
创建时间:
2021-03-19



