Additional file 1 of Proof of concept for multiplex detection of antibodies against Chlamydia species in chicken serum using a bead-based suspension array with peptides as antigens

Additional file 1. Results of the suspension array with sera from mice infected with various Chlamydia species. Pooled sera from mice experimentally infected with Chlamydia, and two pools from naive mice, were tested with bead sets loaded with antigens as indicated. Results for C. psittaci, C. gallinacea, and the last naive mouse are also presented in Figure 1. The results are given in MFI, values above the cutoffs are highlighted in red. For beads with the negative control (NC) compound, values given are the average MFI of three parallel runs; the standard deviations are also given (SD). Serum-specific cutoffs were calculated as three times the average signal on NC beads from three parallel runs (3*avNC). Abbreviations: p, purified peptide; Cga, Cps, Cab, and Cav resp. C. gallinacea, C. psittaci, C. abortus, C. avium. NB in the manuscript normalized data are used for which the cutoffs were subtracted from the acquired MFI values.