Kinetic properties and catalytic efficiencies of Ct43Araf and CtGH43 from C. thermocellum.
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The assays with natural substrates were carried out with 20 mM sodium phosphate buffer (pH 5.7) for aCt43Araf and sodium acetate buffer (pH 5.4) for bCtGH43 at 50°C. The assays were performed in triplicates. The incubation time and other conditions for reducing sugar estimation were as same as described in the Materials and Methods section. cThe assays with synthetic pNP-glycosides were carried out in 20 mM sodium phosphate buffer pH 5.7.
创建时间：
2015-12-02
