Diversity and activity of soil bacterial communities in the rhizophere of poplars from different origins. soil metagenome

The direct isolation of total RNA and DNA from soil samples and its analysis by sequencing with high through-put techniques (454-pyroseqencing) are useful tools for metagenomic and metatranscriptomic analyses. It provides insights into the diversity and the ecosystem function of microbial communities. Furthermore, the metatranscriptomic approach provides an opportunity to detect novel genes, which might encode key functions for important biogeochemical cycles. The aim of this study was to examine the soil structure of bacterial communities in the rhizospheres of poplars from different origins. In addition the seasonal change of the bacterial community composition was investigated. In a second experiment the influence of fertilization on total and active bacterial communities was validated. Thsi Project was funded by the State of Lower Saxony (Ministry of Science and Culture).