ATAC-seq of human plasmacytoid dendritic cells in adult and elderly donors

Aging is associated with profound changes in immune cell function. To investigate how aging impacts plasmacytoid dendritic cells (pDCs), we profiled purified pDCs from healthy adult (20–40 years) and elderly (>65 years) human donors. Using bulk RNA-seq (GSE269411) and bulk ATAC-seq (GSE279911), we identified transcriptional and epigenetic changes that occur with age. These datasets provide a resource to explore age-associated alterations in pDC identity, gene regulation, and chromatin accessibility. pDCs were FACS-sorted from 6 adult (20-40 years old) and 6 elderly (>65 years old). DNA extraction and library preparation was performed by the Stanford Functional Genomics Core. DNA was stored at -20C post transposase reaction until all samples had been collected. Samples were sequenced on a NovaSeq 6000.