Radial topographies of biofilm colonies

During the biofilm life cycle, bacteria attach to a surface and then reproduce, forming crowded, growing communities. Difficulties in accurately measuring biofilm height across relevant time and length scales have prevented testing diverse biophysical models empirically. Using white light interferometry, we measure the heights of microbial colonies with nanometer precision from inoculation to their final equilibrium height, producing a novel and detailed empirical characterization of vertical growth dynamics. This dataset corresponds to the profiles of developing biofilms, measured through white-light interferometry. This dataset represents novel high-resolution characterizations of developing biofilms in the vertical direction, empirical measurements that can be utilized to test models for biofilm development, ranging from bulk-dynamics to surface fluctuations.

在生物膜（biofilm）的生命周期中，细菌首先附着于表面，随后进行繁殖，形成拥挤且不断生长的群落。由于难以在相关的时间与长度尺度上精准测定生物膜高度，学界始终无法通过实验验证各类生物物理模型。本研究采用白光干涉术（white light interferometry），从接种阶段直至生物膜达到最终平衡高度，以纳米级精度测定微生物群落的高度，由此对生物膜的垂直生长动力学实现了新颖且详尽的实验表征。本数据集对应通过白光干涉术测得的发育中生物膜的轮廓数据。本数据集提供了发育中生物膜垂直方向上的新型高分辨率表征数据，这类实验测量结果可用于验证各类生物膜发育模型，涵盖从整体动力学到表面波动的全谱系模型。

搜集汇总

背景与挑战

背景概述

该数据集通过白光干涉测量法，以纳米级精度测量了生物膜菌落从接种到平衡高度的垂直生长动态，提供了高分辨率的经验表征。数据集包含CSV格式的干涉测量数据及相关脚本，可用于测试生物膜发展的生物物理模型，并受美国国家科学基金会等机构资助。