Raw Data CL_DL and Citrus latifolia components (08.12.2025)

This dataset originates from two ethically approved in vivo studies conducted in male Lewis rats using the adjuvant arthritis (AA) model. All procedures complied with EU Directive 2010/63/EU, the European Convention for the Protection of Vertebrate Animals, and institutional 3Rs requirements. Arthritis was induced via intradermal injection of Mycobacterium butyricum. Animals were then treated orally with Citrus latifolia essential oil (20 or 40 mg/kg), D-limonene (25 or 100 mg/kg), sub-therapeutic methotrexate (0.3 mg/kg), or corresponding combination regimens.Primary outcomes included biometric parameters, inflammatory cytokines (IL-17A, IL-1β, MMP-9) and oxidative-stress markers (CAT, SOD, TEAC, 4-HNE), measured using validated plethysmometry, ELISA, and spectrophotometric assays. The volatile constituents of Citrus latifolia essential oil were characterised by GC-MS.The dataset from two in vivo studies performed in adjuvant arthritis rats. This study investigated the anti-arthritic and anti-inflammatory potential of Citrus latifolia (CL) essential oil and its principal component, D-limonene (DL), administered alone and in combination with MTX in the adjuvant arthritis (AA) model in Lewis rats. Moreover, the file contains the volatile components identified in Citrus latifolia essential oil, analysed by GC-MS.