No detectable MRC1 gene expression in Huh7 orthotopic tumors in SCID mice.

No detectable expression of human MRC1 in Huh7 orthotopic xenograft tumors in mice. Mouse Mrc1 mRNA is detectable in Huh7 tumors, albeit at ∼10 fold less than in liver. This expression profile of mannose receptor may contribute, at least in part, to hampered activity of rhASM at the dosing and administration regimen used in the present study. Data represent mean CT values ± standard deviation. Tissues used in sample analyses were from rhASM/sorafenib treated mice. Standard deviation in ‘Primer specificity:’ reflects technical reproducibility. Standard deviation in ‘Sample analyses:’ also reflects variability between animals. CT threshold cycle, − undetermined CT during 40 qPCR cycles.