ARRIVE Guidelines dhani redhono-Author Checklist

<em><strong>Study Design</strong></em> This study is an experimental post-test-only control group, with anthrax animal model, male white rats (<em>Rattus norvegicus</em>) were used as experimental animal subjects due to their anthrax toxin receptors 2 (ANTXR2), which are adaptable, easy to find, and produce many congenital strains. The derivatives of male white rats (<em>Rattus norvegicus</em>) were already used for various purposes in numerous previous studies, such as behavioral studies or medical testing (13,14). This research took place at Indonesian Agency for Agricultural Research and Development (BALITVET), Bogor, Central experimental animals PAU Universitas Gadjah Mada, Yogyakarta, and the Anatomic Pathology Laboratory, Faculty of Medicine, Universitas Sebelas Maret, Surakarta. This study uses eight white male rats (Rattus norvegicus) within each group. The research sample was taken from 40 male white rats (<em>Rattus norvegicus</em>) aged three to four months old, weighing 180–200 g. A standard BR I rat feed was used with the adjusted amount to the average body weight. The allocation of experimental animals into each treatment in a homogeneous study was carried out randomly to maintain internal validity. White male rats (<em>Rattus norvegicus</em>) were selected as test animals, due to its’ genetic similarity to humans and adaptability to the laboratory environment (15). Rats that passed the inclusion criteria for this study were fit white male rats with glowing eyes, bright hair, good appetite, active, aged three to four months, and weighed 180–200 g. While rats that didn’t pass the inclusion criteria were white male rats that are sick, with signs of dimmed eyes, low appetite, dull hair, inactive, and underweight. This study used a complete randomized design and carried out an examination at the end of the study and it use animal models of anthrax which are divided into 5 groups, namely P1, anthrax animal model given propolis 200 mg/kg seven days before induction of anthrax spores for up to 14 days; P2, anthrax animal model and given 200 mg/kg of propolis for 14 days; P3, anthrax animal model and was given propolis 200 mg/kg for seven days; P4, anthrax animal model, were given Amoxicillin 9 mg/kg intravenously and were given propolis 200 mg/kg for 14 days; and the group K, anthrax animal model were not given any treatment.<em><strong>研究设计</strong></em> 本研究为仅后测对照组实验设计，采用炭疽动物模型，选用雄性褐家鼠（<em>Rattus norvegicus</em>）作为实验动物，因其表达炭疽毒素受体2（ANTXR2），且适应性强、易于获取且可大量繁育。此前诸多研究已将雄性褐家鼠应用于行为学研究或医学检测等领域（13,14）。 本研究实施地点包括印尼农业研究与发展局（BALITVET）茂物分院、日惹加查马达大学中央实验动物中心PAU，以及梭罗苏迪尔曼大学医学院病理解剖学实验室。 本研究每组设置8只雄性褐家鼠，实验样本取自40只3~4月龄、体重180~200g的雄性褐家鼠。饲料采用标准BR I大鼠饲料，投喂量根据实验动物平均体重进行调整。为保证研究内部效度，实验动物通过随机分配实现同质性分组。 选择雄性褐家鼠作为实验动物的另一原因在于其与人类的遗传相似性，且对实验室环境具有良好适应性（15）。本研究纳入标准为：健康雄性褐家鼠，眼部明亮、被毛顺滑、食欲良好、活动活跃，月龄3~4个月，体重180~200g；排除标准为患病个体，表现为眼部晦暗、食欲低下、被毛粗糙、活动迟缓以及体重不足。 本研究采用完全随机设计，仅在研究结束时开展检测，所使用的炭疽动物模型分为5组：P1组：炭疽动物模型于炭疽芽孢诱导前7天开始给予200mg/kg蜂胶，持续至诱导后14天；P2组：炭疽动物模型给予200mg/kg蜂胶，持续14天；P3组：炭疽动物模型给予200mg/kg蜂胶，持续7天；P4组：炭疽动物模型静脉给予9mg/kg阿莫西林，同时给予200mg/kg蜂胶，持续14天；K组：炭疽动物模型不施加任何干预。