Transcriptional dynamics of hepatic stellate cells in experimental acute liver injury, liver fibrosis and liver fibrosis reversal.

Transcriptional alterations during different stages of hepatic stellate cell activation were determined using RNA Sequencing. Models include acute liver injury (1 injection of CCl4) and chronic liver injury (8 injections of CCl4 over 4-week period) with different recovery timepoints. Illumina NextSeq 500 High was used for sequencing. Bulk RNA sequencing of isolated mouse hepatic stellate cells after following injury: 4wk CCl4 (8 injections) + 24h (n = 4) 4wk CCl4 (8 injections) + 72h (n = 4) 4wk CCl4 (8 injections) + 14days (n = 4) 1 injection CCl4 + 24h (n = 3) 1 injection CCl4 + 72h (n = 4) 1 injection CCl4 + 7days (n = 4) healthy (non-injected) (n = 4)