b-Catenin and FOXO3 dependent expression profiling in human T-cell acute lymphoblastic leukemia

T-cell acute lymphoblastic leukemia/lymphoma (T-ALL) is a malignancy curable in almost 80% of pediatric and 40% of adult patients. It is a heterogeneous disease characterized by the presence of leukemia stem cells (LSCs) which have been postulated to be responsible for relapses. B-Catenin and FOXO3 modulate LSC activity in T-ALL. To define the gene expression profiling modulated by either b-Catenin and FOXO3 in T-ALL, we generated double knockout FOXO3nullCTNNB1null PF382 cell line of T-ALL using CRISPR/Cas9 technology. Subsequently, FOXO3nullCTNNB1null PF382 cells were co-transduced with an active FOXO3 triple mutant (FOXO3TM) alone or in combination with a stable isoform of b-Catenin (DeltaGSK) or empty vector control to perform RNA-Sequencing (RNA-Seq) assay.