Additional file 19 of Exposure to the gut microbiota drives distinct methylome and transcriptome changes in intestinal epithelial cells during postnatal development

Validation of a subset of differentially expressed and methylated genes. Small intestinal epithelial tissue was harvested by scraping from an independent cohort of GF and CONV-R mice from another gnotobiotic animal facility and both DNA and RNA were isolated for qPCR expression analysis and targeted methylation analysis using amplicon sequencing. Asterisks denote observations in the validation data that showed the same trend/direction as in the initial data, but were only very close to reaching the significance threshold after correction for multiple testing and therefore were considered as validation. (XLSX 48 kb)