Saline soil microbiome study biases caused by different DNA extraction protocols

In the present study, we estimated the bias caused by different DNA extraction methods in saline soil microbiome studies, and we evaluated the microbial diversity and community bias caused only by different physical lysis steps. Also, the bias of amplicon sequencing data processing pipeline, which caused by different DNA extraction methods, was estimated. The bioinformatic processing pipeline included quality filtering, alpha diversity, beta diversity, microbial community composition, phylogenetic analysis, and functional prediction.